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Norovirus: finding its recognition step by step

Norovirus is the leading cause of epidemic and endemic acute gastroenteritis worldwide; it is also the leading cause of foodborne disease outbreaks. This virus genus has many characteristics making it perfect foodborne pathogen including low infectious dose, high transmissibility, environmental persistence and resistance to many commonly used disinfectants and sanitizers.

Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016

The University Hospital of Dijon in France has published an article comparising the performance of seven immunochromatographic kits comercially available in Europe. One of those tests is the Simple Norovirus test from OPERON.

Diagnostic screening for subclinical celiac disease using a rapid test in children aged 2–4, 2015

Dr. María Vega Almazán and co-workers from different Pediatric, Clinical Diagnostic and Research Units from Granada and Cordoba (Spain) published an interesting article studying the prevalence of subclinical celiac disease (CD) and analyzing the diagnostic yield of a new rapid test in children aged 2–4. Our Simple CD2WB immunochromatographic test strip was used in this study being an excellent diagnostic screening tool with high sensitivity and negative predictive value.

HIGH+LOW PAPILLOMASTRIP POSTER

PapillomaStrip test is a test based on the reverse blot technique that allows qualitative detection in DNA samples from cervico-uterine smears or biopsies, of 37 human Papillomavirus subtypes:
Medium-high risk: 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73 and 82 (MM4 e IS39).
Low risk: 6, 11, 40, 42, 43, 44, 54, 61, 62, 67, 70, 71, 72, 74, 81, 83, 84 y 91.

NOROVIRUS POSTER (new version)

The OPERON Norovirus test is a chromatographic immunoassay for the qualitative detection, in separate bands, of Genogroup I (GI) and Genogroup II (GII) of Norovirus in human faeces.

The test is based on the immunological capture of coloured microbeads during its moving along a membrane on which specific monoclonal antibodies against GI and GII have been immobilized in two separate bands.

CLOSTRIDIUM DIFFICILE POSTER (new version)

OPERON's GDH immunochromatographic assay is a rapid test for the qualitative detection of glutamate dehydrogenase (GDH) from Clostridium difficile in human stool samples. A positive signal in the test indicates the presence of C.difficile in the stool sample. Subsequent analysis of the sample with a test for detecting C.difficile toxins (such as OPERON's 2A-Bdiff test) confirms whether the strain is toxigenic and, therefore, pathogenic and disease-causing.

HLA B27 POSTER (two technologies)

B27 OligoGen is a test designed for the qualitative detection of HLA B*27 alleles through the amplification of an specific genomic region and its subsequent identification by oligo-immunochromatography. The procedure of B27 OligoGen kit consists in three steps:
a) DNA extraction
b) amplification by PCR
c) hybridization and developing

RAW MATERIALS POSTER

The necessary MONOCLONAL ANTIBODIES (MAbs) or RECOMBINANT ANTIGENS (rAg) needed in the manufacture of our tests are produced in our facilities.

ROTA-NORO POSTER

The OPERON Rota-Noro chromatographic immunoassay is a procedure for the qualitative detection, in separate bands, of Rotavirus and Norovirus antigens in human faeces.

HLA B5701 POSTER

The HLA B5701 Strip test allows the determination of HLA B*5701 alleles. The procedure of HLA B5701 kit consists in three steps:
a) DNA extraction
b) amplification by PCR
c) hybridization/developing.

HLA B27 STRIP POSTER

The HLA B*27 Strip test allows the determination of the HLA B*27 allele. The HLA B27 Strip kit procedure has three steps:
a) DNA extraction
b) PCR amplification
c) hybridization/development.

LACTOSTRIP POSTER

LactoStrip Test is a test designed for the detection of two MCM6 gene polymorphisms associated with the persistence of lactase activity in adults: C/T 13910 and G/A 22018.

CELIAC DISEASE POSTER

Simple CD1, CD2, CD1WB and CD2WB are immunochromatographic tests designed to detect antibodies (IgA/IgG/IgM) in human serum and human blood respectively, against gliadins and human tissue transglutaminase, the main autoantigen recognized by the anti-endomysial antibodies.

CELIAC STRIP POSTER

The CeliacStrip test is an assay that allows the identification of the main HLA haplotypes associated with celiac disease.
The test detects the presence or absence of haplotypes that encode HLA-DQ2 and HLA-DQ8:

HLA - DQ2 cis: DQA1*05 - DQB1*02 - DRB1*03
HLA - DQ2 trans: DQA1*05 - DQB1*0301 - DRB1*11/DRB1*12 - DQA1*02 - DQB1*02 - DRB1*07
HLA - DQ8: DQA1*03 - DQB1*0302 - DRB1*04

cGMP POSTER

Immunochromatographic test for the qualitative detection of casein glycomacropeptide (cGMP) in milk. The cGMP is produced by enzymatic or bacterial induced degradation of the casein present in milk. This degradation takes place during the cheese manufacturing process, where specific proteases are added to act on the milk casein. Therefore its presence in milk is an indication of milk adulteration with whey.

CHAGAS POSTER

Simple Chagas / Stick Chagas is an immunochromatographic test designed to detect antibodies specific for Trypanosoma cruzi in serum. This protozoan is the causal agent for Trypanosomiasis or Chagas disease.

YCHROMSTRIP POSTER

The YChromStrip test is a test for the detection of deletions in the Azospermia Factor (AZF) located on chromosome Y. These deletions are the cause of male infertility due to azospermia (absence of sperms) or oligospermia (low sperm count).

The test detects the presence or absence of six STSs (sequence tagged sites) located in three different regions of the Azoospermia Factor: AZFa (sY84 and sY86), AZFb (sY127 and sY134) and AZFc (sY254 and sY255).

HYPERdiff for the detection hyper-virulent strains of C.difficile

A new immunochromatographic (IC) rapid tests for the detection of suspected hyper-virulent strains (O27 or O17) of Clostridium difficile von Eichel-Streiber C., Murillo R. and Villacampa M. and tgcBIOMICS GmbH, Carl-Zeiss Str. 51, D-55129 Mainz, Germany OPERON S.A.

Immunodiagnostics, Cuarte de Huerva, Zaragoza, Spain

2A-Bdiff for the detection of toxin A and toxin B of C.

Rapid immunochromatographic point of care test that differentiates Clostridium difficile toxins TcdA & TcdB Murillo R., Villacampa M. and von Eichel-Streiber C. OPERON S.A. Immunodiagnostics, Cuarte de Huerva, Zaragoza, Spain tgcBIOMICS GmbH, Carl-Zeiss Str. 51, D-55129 Mainz, Germany

Cryptosporidium, Giardia lamblia, Entamoeba

Comparison of immunochromatographic assays for detection of Cryptosporidium spp., Giardia duodenalis and Entamoeba Histolytica vs. ELISA, microscopy and PCR.

Celiac Disease

Despistaje de enfermedad celíaca en escolares mediante test rápido en atención primaria.

Blesa L1, López A, Donat E, Grupo de Pediatras de Atención Primaria2, Ribes-Koninckx C

Unidad de Gastroenterología Hospital Infantil La Fe, Valencia. 1 Centro de Salud Serrería II, Valencia.

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