These tests are based on the capture with specific probes of nucleic acids of amplified fragments by the polymerase chain reaction.
This capture can be done with immobilised probes either on to a membrane (OpeGen line, based on the Southern reverse blot) or with probes bounded to coloured latex particles (OligoGen line, based on the oligo-immunocromatography).
One of the guidelines of good laboratory practices in Molecular Biology recommends the availability of independent rooms for reagent preparation, DNA extraction, PCR preparation, and amplification. The Molecular Biology area in Operon S.A. is divided into 5 independent laboratories, with controlled access, and a positive pressure and UV irradiation system, minimizing the risk of contamination with PCR amplicons and ensuring the quality of every manufactured product.
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